Background[ edit ] Molecular cloning is one of the most commonly used procedures in molecular biology. A gene of interest may be inserted into a plasmid vector via ligationand the plasmid is then transformed into Escherichia coli cells. However, not all the plasmids transformed into cells may contain the desired gene insert, and checking each individual colony for the presence of the insert is time-consuming, therefore a method for the detection of the insert would be useful for making this procedure less time- and labor-intensive. One of the early methods developed for the detection of insert is blue-white screening which allows for identification of successful products of cloning reactions through the colour of the bacterial colony.
June 20, by ToolsTutorials 29 Comments Selecting fly-away hair can be one of the toughest assignments in Photoshop. The Extract command can be helpful. Third-party plug-ins, such as Mask Pro www.
But sometimes the easiest way to select those random strands of hair is right in your Channels palette. Open the image in Photoshop.
Open the Channels palette. Click on each channel, one at a time, to find the channel with the greatest contrast between the strands of hair and the background behind them. In our sample image PhotoSpin. With the Blue copy channel active in the Channels palette, we can use Levels or Curves to maximize the contrast.
Using the Lasso tool and the Fill command, in combination with the Brush tool and the Eraser tool, we can easily fill in the subject and delete the remaining areas of the background.
In the upper window, only the Blue copy channel is active and visible. In the lower window, only the Blue copy channel is active, but all channels are visible.
When the mask is complete, click on the composite channel at the top of the Channels palette to make it active, then Command-click Mac or Control-click Windows on the mask channel Blue copy, in this example to load the channel as a selection.
You can delete the background, fill it with color, or invert the selection and copy the subject, ready to paste into another image. This entry was posted in ToolsTutorials.BEST THRIFT STORES IN THE USA. Each day at all Red, White and Blue Thrift Stores, we have sales to keep our items moving and keep our customers happy.
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